human il17 / il17a protein  (Sino Biological)

Journal: International Journal of Molecular Medicine

Article Title: IL-37/IL-1R8 blocks keratinocyte acantholysis via suppressing ADAM17/EGFR

doi: 10.3892/ijmm.2026.5793

Figure Lengend Snippet: IL-37 protects HaCaT cells from Dsg3-induced keratinocyte dissociation and apoptosis. (A) The effect of different dilutions of Dsg-3 antibodies on cell dissociation was analyzed by immunofluorescence. (B) HaCaT cells were treated with different concentration of IL-37 recombinant protein (0, 25, 50, 100, and 200 ng/ml). Cell viability was detected by CCK-8 assay. (C) HaCaT cells were treated with the Dsg-3 antibodies, followed by administration of the IL-37 recombinant protein. The concentration of IL-37 was analyzed using an ELISA assay. (D) The relative mRNA expression of IL-37 was detected using RT-qPCR. The (E) concentration and (F) relative mRNA expression of IL-10 were measured using an IL-10 ELISA kit and RT-qPCR, respectively. The (G) oncentration and (H) relative mRNA expression of IL-6 were measured by the IL-6 ELISA kit and RT-qPCR, respectively. The (I) concentration and (J) relative mRNA expression of IL-17 were measured by the IL-17 ELISA kit and RT-qPCR, respectively. & P<0.05; * P<0.05 vs. Control group; # P<0.05 vs. anti-Dsg3 group. Data are presented as mean ± SD, n=3 biological independent replicates. One-way ANOVA with Bonferroni's post-hoc test was used for multiple group comparisons. IL, interleukin; Dsg3, desmoglein-3; ELISA, enzyme-linked immunosorbent assay; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Subsequently, the cells were treated with IL-37 recombinant protein (isoform b; 100 ng/ml; cat. no. 10155-HNAE; Sino Biological, Inc.) for 24 h at 37°C.

Techniques: Immunofluorescence, Concentration Assay, Recombinant, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Medicine

Article Title: IL-37/IL-1R8 blocks keratinocyte acantholysis via suppressing ADAM17/EGFR

doi: 10.3892/ijmm.2026.5793

Figure Lengend Snippet: IL-37/IL-1R8 protects HaCaT cells from keratinocyte dissociation and apoptosis through the ADAM17/EGFR pathway. (A) The interaction between IL-37 and IL-1R8 was analyzed using co-immunoprecipitation. (B) HaCaT cells were transfected with IL-1R8 siRNA and the transfection efficiency were detected by western blotting. (C) HaCaT cells were transfected with IL-18Rα siRNA and the transfection efficiency were detected by western blotting. (D) IL-1R8 siRNA or IL-18Rα transfected HaCaT were treated with anti-Dsg-3 and IL-37 recombinant protein. The number of fragments was analyzed by cell dissociation assay. (E and F) Cell apoptosis was analyzed by flow cytometry. (G) Protein expression levels of Bcl-2 and Bax were determined by western blotting. & P<0.05 vs. si-NC; * P<0.05 vs. anti-Dsg3 + IL-37 + si-NC group. Data are presented as mean ± SD, n=3 biological independent replicates. One-way ANOVA with Bonferroni's post-hoc test was used for multiple group comparisons. IL, interleukin; ADAM17, TNF-alpha-converting enzyme; EGFR, epidermal growth factor receptor; si, short interfering.

Article Snippet: Subsequently, the cells were treated with IL-37 recombinant protein (isoform b; 100 ng/ml; cat. no. 10155-HNAE; Sino Biological, Inc.) for 24 h at 37°C.

Techniques: Immunoprecipitation, Transfection, Western Blot, Recombinant, Flow Cytometry, Expressing